化学性质
Descriptions
PreparationNoteEquivalenttofirst40%ammoniumsulfatefractionofMandl,I.,etal.,J.Clin.Invest.,32,1323(1953).
QualityAlsocontainsclostripain,nonspecificneutralprotease,andtrypticactivities.
UnitDefinitionOnecollagendigestionunitliberatespeptidesfromcollagenequivalentinninhydrincolorto1.0μmoleofleucinein5hratpH7.4at37°Cinthepresenceofcalciumions.
OneFALGPAhydrolysisunithydrolyzes1.0μmoleoffurylacryloyl-Leu-Gly-Pro-AlaperminatpH7.5at25°Cinthepresenceofcalciumions.
Oneneutralproteaseunithydrolyzescaseintoproducecolorequivalentto1.0μmoletyrosineper5hratpH7.5at37°C.
Oneclostripainunithydrolyzes1.0μmoleofBAEEperminatpH7.6at25°CinthepresenceofDTT.
Properties
formessentiallysalt-free,lyophilizedpowder
storagetemp.−20°C
