化学性质
GeneraldescriptionCollagenisclassifiedintoanumberofstructurallyandgeneticallydistincttypes.WeusethenomenclatureproposedbyBornsteinandTraub.DonotconfuseSigmatypedesignationswithrecognizedcollagenclassificationtypes.
PreparationNoteRecommendedforuseasacellculturesubstratum.Maynotbesuitablefor3-Dgelformation.
Sterilesolutionpreparedfromrattailtendonsbyamodificationofthepublishedextractionmethod.Itissuppliedasanaqueoussolutionin20mMaceticacidwithaproteinconcentrationasstatedonthebottle.(approx.100mgproteinpervial).ProteinconcentrationwasestimatedbytheBiuretmethod.
SDSpolyacrylamidegelelectrophoresisanalysisshowsthetypicalbandpatternforTypeIcollagen,withadoubletatapparentmolecularweightsof115and130kDaandanotherdoubletat215and235kDa.Basedonthisanalysis,thepurityofthecollagensampleis>95%.
