General description Collagen is classified into a number of structurally and genetically
distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse
Sigma type designations with recognized collagen classification types.
Preparation Note Recommended for use as a cell culture substratum. May not be suitable
for 3-D gel formation.
Sterile solution prepared from rat tail tendons by a modification of the published extraction
method. It is supplied as an aqueous solution in 20mM acetic acid with a protein
concentration as stated on the bottle. (approx.100mg protein per vial). Protein
concentration was estimated by the Biuret method.
SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I
collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another
doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample