西亚试剂：：Ethanol Precipitation of DNA

Precipitation of DNA
This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples.

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Materials

3M Sodium Acetate buffer, pH 5.2 (store at 4 °C)
Cold 100% Ethanol (-20°C)
Cold 70% Ethanol in sterile dH2O (-20°C)
DNA sample
4 °C Microcentrifuge (normal microcentrifuge in cold room works fine). All centrifugations should be on "soft" (no brake) setting.
 

Procedure

Transfer DNA to a container where it fills one fourth the total volume (a 500 µL tube should have no more than 125 µL of DNA solution, for example)
Add one tenth volume of Sodium Acetate buffer to equalize ion concentrations
Add at least two volumes of cold 100% ethanol; let stand in -20°C freezer for at least one hour
Centrifuge sample for 15 minutes at highest speed in a 4°C microcentrifuge
Remove as much supernatant as possible with a 1 mL micropipet; recentrifuge, then remove the rest with a 200 µL pipet
Add 200 µL of cold 70% ethanol; centrifuge for 5 minutes in a 4 °C centrifuge
Remove supernatant with a 200 µL pipet; evaporate remaining ethanol in a 37 °C water bath
Resuspend pellet in desired volume of water or TE buffer