西亚试剂：Genomic DNA Quickprep for PCR

macerate tissue in Eppendorf tube without butter at RT
add 400 ml extraction buffer
vortex for 4 sec
leave sample at RT until other samples are ready (> 1 h)
spin in microfuge for 1 min
transfer 300 ml of supernatant to different Eppendorf tube (prefilled with 300 ml isopropanole)
mix and leave at RT for 2 min
spin for 5 min
vacuum dry pellet and take up in 100 ml TE
use 1-2.5 ml for PCR

DNA is stable for one year at 4°C


Solutions:

Extraction buffer: `200 mM Tris-HCl pH 7.5 250 mM NaCl 25 mM EDTA 0.5% SDS`

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