西亚试剂：The cspA mRNA Is a Thermosensor that Modulates Translation

The cspA mRNA Is a Thermosensor that Modulates Translation of the Cold-Shock Protein CspA

Anna Maria Giuliodori, Fabio Di Pietro, Stefano Marzi, Benoit Masquida, Rolf Wagner, Pascale Romby, Claudio O. Gualerzi, Cynthia L. Pon

Cold induction of cspA, the paradigm Escherichia coli cold-shock gene, is mainly subject to posttranscriptional control, partly promoted by cis-acting elements of its transcript, whose secondary structure at 37°C and at cold-shock temperature has been elucidated here by enzymatic and chemical probing. The structures, which were also validated by mutagenesis, demonstrate that cspA mRNA undergoes a temperature-dependent structural rearrangement, likely resulting from stabilization in the cold of an otherwise thermodynamically unstable folding intermediate. At low temperature, the “cold-shock” structure is more efficiently translated and somewhat less susceptible to degradation than the 37°C structure. Overall, our data shed light on a molecular mechanism at the basis of the cold-shock response, indicating that cspA mRNA is able to sense temperature downshifts, adopting functionally distinct structures at different temperatures, even without the aid of trans-acting factors. Unlike with other previously studied RNA thermometers, these structural rearrangements do not result from melting of hairpin structures.

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