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Generation of Long RNA Chains in Water*
Giovanna Costanzo?,1, Samanta Pino§,1, Fabiana Ciciriello§ and Ernesto Di Mauro?,2
From the ?Istituto di Biologia e Patologia Molecolari, CNR,
the §Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza di Roma, and
the ?Fondazione Istituto Pasteur-Fondazione Cenci-Bolognetti, c/o Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza di Roma, P. le Aldo Moro, 5, Rome 00185, Italy
The synthesis of RNA chains from 3′,5′-cAMP and 3′,5′-cGMP was observed. The RNA chains formed in water, at moderate temperatures (40–90 °C), in the absence of enzymes or inorganic catalysts. As determined by RNase analyses, the bonds formed were canonical 3′,5′-phosphodiester bonds. The polymerizations are based on two reactions not previously described: 1) oligomerization of 3′, 5′-cGMP to ～25-nucleotide-long RNA molecules, and of 3′,5′-cAMP to 4- to 8-nucleotide-long molecules. Oligonucleotide A molecules were further extended by reciprocal terminal ligation to yield RNA molecules up to >120 nucleotides long and 2) chain extension by terminal ligation of newly polymerized products of 3′,5′-cGMP on preformed oligonucleotides. The enzyme- and template-independent synthesis of long oligomers in water from prebiotically affordable precursors approaches the concept of spontaneous generation of (pre)genetic information.
