西亚试剂：Deep surveying of alternative splicing complexity in the hu

Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing

Qun Pan1, Ofer Shai1,2, Leo J Lee1,2, Brendan J Frey1,2 & Benjamin J Blencowe1,3

We carried out the first analysis of alternative splicing complexity in human tissues using mRNA-Seq data. New splice junctions were detected in 20% of multiexon genes, many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data, we estimate that transcripts from 95% of multiexon genes undergo alternative splicing and that there are 100,000 intermediate- to high-abundance alternative splicing events in major human tissues. From a comparison with quantitative alternative splicing microarray profiling data, we also show that mRNA-Seq data provide reliable measurements for exon inclusion levels.

1 Banting and Best Department of Medical Research, University of Toronto, Toronto M5S 3E1, Canada.
2 Department of Electrical and Computer Engineering, University of Toronto, Toronto M5S 3G4, Canada.
3 Department of Molecular Genetics, University of Toronto, Toronto M5S 3E1, Canada

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