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L Broth Agar
1 L of L Broth
15 g agar
Autoclave
L Broth Top Agar
Make L Broth Agar except substitute 7.5 g agar/L.
TMG (10 mM Tris-HCl, pH 7.5; 10 mM MgSO4; 0.1%
For 100 ml:
1 ml of 1M Tris-HCl pH 7
1 ml of 1M MgS04
0.1 g gelatin
QS to 100 ml, and autoclave