西亚试剂：：Identification of miRNAs and miRNA-mediated

Plant microRNAs (miRNAs) post-transcriptionally regulate target gene expression to modulate growth and development and biotic and abiotic stress responses. By analyzing small RNA deep sequencing data in combination with the genome sequence， we identified 75 conserved miRNAs and 11 novel miRNAs. Their target genes were also predicted. For most conserved miRNAs， the miRNA-target pairs were conserved across plant species. In addition to these conserved miRNA-target pairs， we also identified some papaya-specific miRNA-target regulatory pathways. Both miR168 and miR530 target the Argonaute 1 gene， indicating a second autoregulatory mechanism for miRNA regulation. A non-conserved miRNA was mapped within an intron of Dicer-like 1 (DCL1)， suggesting a conserved homeostatic autoregulatory mechanism for DCL1 expression. A 21-nt miRNA triggers secondary production from its target genes， nucleotide-binding site leucine-rich repeat protein genes. Certain phased-miRNAs were processed from their conserved miRNA precursors， indicating a putative miRNA evolution mechanism. In addition， we identified a Carica papaya-specific miRNA that targets an ethylene receptor gene， implying its function in the ethylene signaling pathway. This work will also advance our understanding of miRNA functions and evolution in plants.

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